On Diluent and Dilution Method in Hemagglutination Inhibition Test for Human Chorionic Gonadotropin
نویسندگان
چکیده
منابع مشابه
An improved diluent for rubella hemagglutination and hemagglutination-inhibition tests.
Rubella hemagglutinating (HA) antigen was prepared in BHK-21 tissue as 5% cell suspensions and from unconcentrated and 20x concentrated infected supernatant fluids. In some instances, unconcentrated fluids were treated with Tween 80 and ether; cell suspensions were treated with ether alone. Preparations were tested for HA activity in dextrose-gelatin-Veronal (DGV) buffer solutions; 0.85% NaCl; ...
متن کاملBetween-method variation in human chorionic gonadotropin test results.
BACKGROUND Results on sera and calibrators vary 1.4- to 2.3-fold among commercial human chorionic gonadotropin (hCG) assays. The relative contributions of calibrators, standards, hCG charge isoforms, and major structural variants to this variation have not been quantified. METHODS Purified hCG was separated by isoelectric focusing into four fractions with pI ranges of 3-4, 4-5, 5-6, and 6-7. ...
متن کاملHuman Chorionic Gonadotropin
A highly active preparation of human chorionic gonadotropin has been obtained by simple methods of column chromatography on diethylaminoethyl Sephadex and Sephadex G-100. The preparation appeared homogeneous by disc and immunoelectrophoresis, ultracentrifugation, and end group analysis. It assayed at 12,000 i.u. per mg by the rat prostrate gland and accessory sex organ method. The ~20,~ value w...
متن کاملImmunoassay for Human Chorionic Gonadotropin Based on Glassy Carbon Electrode Modified with an Epitaxial Nanocomposite
A highly sensitive electrochemicalimmunosensor was developed to detect hCG based on immobilization ofhCG-antibody (anti-hCG) onto robust nanocomposite containing Gr, Chit,1-methyl-3-octyl imidazolium tetra fluoro borate ionic liquid (IL)(Gr-IL-Chit). AuNPs were used to immobilize hCG antibody on the modifiedelectrode. The amine groups of the antibody are cova...
متن کاملStandardization of assays for human chorionic gonadotropin.
The unsatisfactory standardization of many immunoassays is fairly well recognized, but the problem is hard to tackle (1 ). Recently, studies on the performance of immunoassays for testosterone in female serum (2 ) and for urinary cortisol (1 ) have revealed that the quality of some widely used assays is totally unsatisfactory. The quality of assays for human chorionic gonadotropin (hCG) has als...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1969
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.16.399